Fig. 7.

Memory T-cell responses specific to the peptides N46, N47 or N48 by intracellular staining. PBMCs from SARS-recovered donors were incubated with the indicated peptides (N46, N47 or N48) for 6 h. The intracellular staining for IFN-γ was performed. CD4⁺ and CD8⁺ T cells were gated and analyzed for IFN-γ expression by flow cytometry (A). Results shown are representative of 3 independent experiments from 3 SARS patients. Similar results were also obtained in other experiments for CD8⁺ (B) and CD4⁺ (C) T cells.