Table 1.
Primers used to evaluate cytokine expression by Real-time qPCR in naturally FIV-infected cats treated with rFeIFN protocols.
| Gene | Oligo | Sequence (5′−3′) | Reference |
|---|---|---|---|
| Β-Actin | For | GACTACCTCATGAAGATCCTCACG | Scott et al., 2011 |
| Rev | CCTTGATGTCACGCACAATTTCC | ||
| IL-1β | For | ATTGTGGCTATGGAGAAACTGAAG | Scott et al., 2011 |
| Rev | TCTTCTTCAAAGATGCAGCAAAAG | ||
| IL-4 | For | CCCCTAAGAACACAAGTGACAAG | Taglinger, Van Nguyen, Helps, Day, & Foster, 2008 |
| Rev | CCTTTGAGGAATTTGGTGGAG | ||
| IL-6 | For | GTGTGACAACTATAACAAATGTGAGG | Scott et al., 2011 |
| Rev | GTCTCCTGATTGAACCCAGATTG | ||
| IL-10 | For | ACTTTCTTTCAAACCAAGGACGAG | Scott et al., 2011 |
| Rev | GGCATCACCTCCTCCAAATAAAAC | ||
| IL12p40 | For | TGGCCTTCTGAAGCGTGTTG | Scott et al., 2011 |
| Rev | GAAGTACACAGTGGAGTGTCAGG | ||
| IFN-γ | For | TGCAAGTAATCCAGATGTAGCAG | Taglinger et al., 2008 |
| Rev | GTTTTATCACTCTCCTCTTTCCAG | ||
| TNF-α | For | CACATGGCCTGCAACTAATC | Taglinger et al., 2008 |