Table 1.

T-cell IFN-γ ELISpot positive N-peptide sequences

N peptides			IFN-γ SFC/106 splenocytes (mean ± SD)
Number	N-aa	Sequence	p-hLAMP-N	p-N	N-GST
11	76–93	NTNSGPDDQIGYYRRATR	57 ± 2	3 ± 2	6 ± 1
12	80–99	GPDDQIGYYRRATRRVRGGD	429 ± 8	266 ± 18	46 ± 12
13	84–101	QIGYYRRATRRVRGGDGK	377 ± 11	209 ± 6	55 ± 6
14	92–109	TRRVRGGDGKMKELSPRW	309 ± 13	212 ± 7	24 ± 5
15	100–114	GKMKELSPRWYFYYL	75 ± 12	55 ± 7	9 ± 2
21	130–149	GIVWVATEGALNTPKDHIGT	106 ± 4	61 ± 8	5 ± 3
35	241–258	QQQGQTVTKKSAAEASKK	131 ± 20	23 ± 3	2 ± 1
51	352–366	ILLNKHIDAYKTFPP	137 ± 10	34 ± 8	38 ± 4
52	353–370	LLNKHIDAYKTFPPTEPK	118 ± 11	50 ± 8	46 ± 11

All ELISpot responses were to a group of 9 dominant peptides, shown by peptide number, amino acid region in N, sequence, and number of IFN-γ-positive cells of mice immunized with the three immunogens. A cluster (N_76–114) of 5 peptides of 15 to 20 aa, overlapping by 10 to 16, contains 3 of the strongest dominant epitopes.