. 2006 Jan 4;347(1):127–139. doi: 10.1016/j.virol.2005.11.042

Table 2.

Correlation of ELISpot-positive peptides and computational predictions of N-specific H2^d binding motifs

IFN-γ N peptides	PRED^BALB/c H2^d alleles
IFN-γ N peptides	H2-K^d	H2-L^d	H2-D^d	I-A^d	I-E^d
₇₆NTNSGPDDQIGYYRRATR₉₃	–	–	SGPDDQIGY	–	–
₈₀GPDDQIGYYRRATRRVRGGD₉₉	YYRRATRRV	GPDDQIGYY	IGYYRRATR	GYYRRATRR	GYYRRATRR
₈₀GPDDQIGYYRRATRRVRGGD₉₉			GYYRRATRR
₈₄QIGYYRRATRRVRGGDGK₁₀₁	YYRRATRRV	–	IGYYRRATR	GYYRRATRR	RRVRGGDGK
₈₄QIGYYRRATRRVRGGDGK₁₀₁			GYYRRATRR
₉₂TRRVRGGDGKMKELSPRW₁₀₉	–	–	GGDGKMKEL	–	RRVRGGDGK
					VRGGDGKMK
					GKMKELSPR
₁₀₀GKMKELSPRWYFYYL₁₁₄	ELSPRWYFY	LSPRWYFYY	LSPRWYFYY	–	GKMKELSPR
₁₀₀GKMKELSPRWYFYYL₁₁₄		SPRWYFYYL			LSPRWYFYY
₁₃₀GIVWVATEGALNTPKDHIGT₁₄₉	ALNTPKDHI	VWVATEGAL	–	EGALNTPKD	WVATEGALN
₁₃₀GIVWVATEGALNTPKDHIGT₁₄₉					TEGALNTPK
₂₄₁QQQGQTVTKKSAAEASKK₂₅₈	–	–	–	QQGQTVTKK	–
				GQTVTKKSA
				VTKKSAAEA
				TKKSAAEAS
₃₅₂ILLNKHIDAYKTFPP₃₆₆	LLNKHIDAY	–	–	NKHIDAYKT	LLNKHIDAY
₃₅₂ILLNKHIDAYKTFPP₃₆₆	NKHIDAYKT
₃₅₃LLNKHIDAYKTFPPTEPK₃₇₀	LLNKHIDAY	–	–	NKHIDAYKT	LLNKHIDAY
₃₅₃LLNKHIDAYKTFPPTEPK₃₇₀	NKHIDAYKT

The N-specific H2^d binding motifs were analyzed by the PRED^BALB/c system. Each of the IFN-γ ELISpot peptides contained multiple nine amino acids binding motifs that were within the top 5% of all binding scores to the five H2^d alleles. All of the IFN-γ ELISpot peptides except N_241–258 scored were predicted to contain class I binding motifs. Similarly, class II binding motifs were predicted in all except N_76–93 and N_130–149.