Fig. 4.

Involvement of JNK and p38 MAPK activation in AKAV-induced apoptosis. (A) Mock-infected (−) or AKAV-infected (+) Vero E6 cells were not treated (−) or treated (+) with SP600125 (JNK inhibitor) and SB203580 (p38 MAPK inhibitor) for 36 h. Phosphorylation of c-Jun and HSP27 were determined by Western blotting. (B) Vero E6 cells infected with AKAV were not treated or treated with SP600125 and SB203580 for 48 h. (C) Western blot analysis with anti-cleaved caspase-3 antibody was performed using whole-cell extracts from the cells mock-infected (−) or infected (+) with AKAV in the absence (−) or presence (+) of SP600125 and SB203580 for 48 h.