Table 1.
Dual labeling of IBV N protein in Vero and CEK cells indicated a significant linear increase in the 32P:3H ratio (P < 0.0001) of IBV N protein with the time post-infection
Time of harvest: hours post-infection | Chicken embryo kidney cellsa | Vero cellsa |
---|---|---|
7 | 0.35 ± 0.004 | 0.24 ± 0.004 |
9 | 0.83 ± 0.036 | 0.45 ± 0.003 |
11 | 0.80 ± 0.074 | 0.84 ± 0.001 |
13 | 0.73 ± 0.017 | 0.85 ± 0.056 |
16 (Virion N) | 1.22 ± 0.004 | 2.52 ± 0.006 |
Represents cpm ratios of 32P:3H ± standard deviation.