Table 1.
Characteristics of highly pathogenic avian viruses used in this study.
| Virus | Abbreviation | Date of specimen collection | Province | Status of host | H5 HA gene clade | Log10 EID50/mL | Log10 PFU/ml in MDCK cells | Plaque size in MDCK cellsa | MLD50 (log10 EID50) |
|---|---|---|---|---|---|---|---|---|---|
| A/Pigeon/Thailand/VSMU-7-NPT/2004 | Pigeon04 | 13/2/2004 | Nakornpatom | Dead | 1 | 7.6 | 7.2 | Pinpoint (76%) | 0.8 |
| Medium (24%) | |||||||||
| A/Tree sparrow/Ratchaburi/VSMU-16-RBR/2005 | T.sparrow05 | 3/5/2005 | Ratchaburi | Live | 1 | 7.9 | 7.1 | Pinpoint (6%) | 2.2 |
| Medium (72%) | |||||||||
| Large (22%) |
Pinpoint plaque, < 1 mm in diameter; medium plaque, 1–2 mm in diameter; large plaque, > 2 mm in diameter.
a
Plaque size was measured in MDCK cells infected with HPAI viruses at 3 day post-infection.