Table 3.
Nucleotide and amino acid changes in spike following passage of TCoV in embryonating turkey eggs.
| Strain and passage no. | Nucleotidea, Amino Acidb changes | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCoV/TX-1038/98 Pass 40c | C183T | A189C | T193C, Ser65Pro | C198T | C225T | C252A, Asn84Lys | G2269A, Val757Ile | |||||||||
| TCoV/TX-1038/98 Pass 49 | T237C | A252C, Lys84Asn | A271T, Thr91Ser | G380A, Gly127Asp | A403C, Asn135 His | |||||||||||
| TCoV/TX-1038/98 Pass 50 | C237T | C246T | C252T | C403A, His135Asn | C411T | T413C, Ile138 Thr | ||||||||||
| TCoV/TX-1038/98 Pass 52 | T246C | T252C | T271A, Ser91Thr | G379A | A380G, Asp127Ser | A403C Asn135His | T411C | C413T, Thr138Ile | A716G, Gln239Arg | |||||||
| TCoV/TX-R/98 Pass 24d | G416A, Gly139Asp | G540T, Gln180His | ||||||||||||||
a
Positions are relative to the ATG start site in the spike gene with the A being number 1, and the change is synonymous if no amino acid change is indicated.
b
Positions are relative to the beginning of the spike glycoprotein with methionine being number 1.
c
Using passage 1 as the base sequence, no changes were observed for passages 8, 10, 13, 14, 17, 18, 20, and 30.
d
Using passage 1 as the base sequence, no changes were observed for passage 20 and no additional changes from passage 24 were observed for passage 27 and passage 30.