Table 8.

Primer pairs used in RT-PCR reactions to sequence the 3′ end of TCoV isolates passaged in embryonating eggs.

Primer pairs	Locationsa	Sequence
TCoV S1 5′	20,338–20,358	5′-AAGAGTGGCAAGTTGTTAGTT-3′
TCoV S1 3′	22,017–22,037	5′-CCATAGCTGCAAATAGACCTA-3′
TCoV 1511 5′	21,882–21,901	5′-CTGCATCTTGTCGGTTAGCC-3′
TCoV 3809 3′	24,179–24,198	5′-TGCTCACCAGTTTCAATAAT-3′
TCoV 3565 5′	23,936–23,956	5′-ACTTTTGATAATGATGTGGTG-3′
TCoV 5538 3′	25,963–25,983	5′-GCTCTGCTTGTCCTGCTTTGT-3′
TCoV 5046 5′	25,378–25,397	5′-AAGAGTATTTATTTTGAGGA-3′
Oligo-dT 3′	27,621–27,638	5′-TTTTTTTTTTTTTTTTTT-3′

^aPrimer pair nucleotide locations are based on the sequence for TCoV/TX-GL/01 (accession number GQ427174).