Fig. 3.

CPV contacts with recruited mitochondria and Golgi stacks. (A, C and D) Computational tomographic slices (~ 1.2 nm) and (B) semi-thick (~ 200 nm) section visualized by TEM. (A) Side view of a CPV completely surrounded by mitochondria. Holes or channels in the CPV rigid membrane (dashed circle) apparently open the CPV to the cytoplasm. (B) Characteristic RUBV factory with a CPV containing rigid membranes (the star labels one of them) surrounded by RER, mitochondria and Golgi. The Golgi stack approaches laterally to the CPV but no clear contacts are detected. (C and D) Computational tomographic slice of a similar factory showing contacts between Golgi vesicles and the CPVs (arrows). Arrowheads in B and C point to filaments around the factories. RER, rough endoplasmic reticulum; mi, mitochondria. Bars, 200 nm in A to C; 100 nm in D.