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. 2010 Jul 23;405(2):579–591. doi: 10.1016/j.virol.2010.06.043

Fig. 6.

Fig. 6

Immunogold labeling of CPVs. (A and B) correspond to Tokuyasu cryosections while (C and D) are Lowicryl resin sections. (A) Labeling with an antibody specific for the mitochondrial protein p32 showing signals in the CPVs' internal membranes (white arrows) and in the gap between the CPV and the RER (black arrows). (B) Higher magnification view of a CPV labeled with anti-p32 antibodies showing intense signals in CPV's internal membranes (white arrows) and around the CPV (black arrows). (C) Double labeling with a mixture of two mouse monoclonal antibodies specific for lysosomal markers (LAMP1 and LAMP2, white arrowheads) and a rabbit polyclonal antibody specific for P150 protein, one of the two components of the RUBV replicase complex (black arrows). Labeling concentrates in the interior of CPVs. (D) Immunogold labelling with an antibody specific for Mitofusin 2 (MFN2) (mainfield and inset). A weak signal is observed on the internal periphery of CPVs (arrows). Bars, 200 nm in A, C and D; 100 nm in B and inset in H.