Fig. 2.

Cellular localizations of 8a and 8b in SARS-CoV-infected cells and Vero E6 cells transfected with DNA constructs for expressing 8a, 8b and 8ab. Specific mouse anti-8a and anti-8b polyclonal antibodies were used in indirect immunofluorescence experiments to determine the expressions of (A) 8a and (B) 8b, respectively. The top two panels showed the specific reactivities of the anti-8a and anti-8b antibodies to proteins expressed in SARS-CoV-infected cells (right panels) as no unspecific staining was observed for the mock-infected cells (left panels). The bottom two panels showed the reactivities of the antibodies to 8a and 8ab (A) or 8b and 8ab (B) expressed in Vero E6 by transfection of cDNA constructs.