Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Jul 31;354(1):132–142. doi: 10.1016/j.virol.2006.06.026

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006 Elsevier Inc. All rights reserved.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

PMC Copyright notice

Fig. 4 — Effects of 8b on the expression of the small structural protein, E. (A) 293T cells were co-transfected with 2 μg of pXJ-E and 0.1 μg of pXJ-myc-GST (lane 1), 1 μg of pXJ-8a-myc (lane 2), pXJ-8b-myc (lane 3), pXJ-8ab-myc (lane 4), or decreasing amount of pXJ-8b-myc (lanes 5–10). Total cell lysates were subjected to Western blot analysis to determine the expression of E (middle panel) and myc-tagged proteins (top panel). Equal amounts of cells were used in each lane as verified by the level of endogenous actin (bottom panel). (B) 293T cells were co-transfected with 1 μg of pXJ-S and 1 μg of either pXJ-8b-myc or empty vector (lanes 1 and 2). Total cell lysates were subjected to Western blot analysis to determine the expression of 8b-myc (top panel) and S (lower panel). Equal amounts of cells were used in each lane as verified by the level of endogenous actin (middle panel). Similar experiments were performed with 1 μg of pXJ-M-HA (lanes 3 and 4), 0.25 μg of pXJ-N (lanes 5 and 6), 0.4 μg of pXJ-3a (lanes 7 and 8) or 0.4 μg of pXJ-7a (lanes 9 and 10). (C) Vero E6 or 293T cells were co-transfected with 2 μg of pXJ-E and 1 μg of pXJ-8b (lanes 1 and 3) or 1 μg of empty vector (lanes 2 and 4). Total cell lysates were subjected to Western blot analysis to determine the expression of E (top panel) and 8b (middle panel). Equal amounts of cells were used in each lane as verified by the level of endogenous actin (bottom panel). (D) Indirect immunofluorescence experiments were performed to determine the cellular localization of 8b and E in Vero E6 cells co-transfected with pXJ-8b and pXJ-E. The expression of 8b is represented by FITC staining (left panel), whereas the expression of E is represented by rhodamine staining (middle panel). The merged images showed that the 8b and E partially colocalized in co-transfected cells (right panel).