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. 2006 Jul 31;354(1):132–142. doi: 10.1016/j.virol.2006.06.026

Fig. 6.

Fig. 6

Interaction between E and 8b in SARS-CoV-infected cells determined by co-immunoprecipitation experiment. Lysates from mock-infected or SARS-CoV-infected cells were immunoprecipitated using rabbit anti-8b polyclonal antibody (lanes 4 and 6) or an irrelevant antibody (rabbit anti-HA polyclonal antibody, lanes 3 and 5) and protein A-agarose beads. Western blot analyses were then performed to determine the amount of E (upper panel) or 8b (lower panel) present in the lysates before immunoprecipitation (lanes 1 and 2) and the immunocomplexes on the protein A-agarose beads (lanes 3–6).