Table 3.

Biochemical properties of polyomavirus agnoprotein.

	BKPyV	JCPyV	SV40	SV40Ri257	SA12	ChPyV-Az	ChPyV-Ta	ChPyV-Bob	SqPyV	BPyV	SLPyV	MyPV	MaPyV
aa	66	71	61	118	68	64	65	76	70	114	50	30	154
kD	7.4	8.1	7.3	10.3	7.8	7.3	7.5	8.8	7.8	13.2	5.3	3.7	17.6
pI	10.0	10.1	10.1	9.7	9.8	11.2	10.9	11.6	10.4	4.6	10.1	12.0	7.6
DNA binding			ss, ds
Phosphorylation	S7,11,52,62,66 T21	S7,11,15,55 T21 Y62	S7,11,17,21 T19,45,57,62	S7,11,17, 62 T19,21,45,56
Acetylation	K60,64	K9,13,69	K13,59	K13	K52,65,66		K65	K62	K4		K2,3,50		K105
Methylation	K22,R41	K9,22	K51		K50,51		K8,60	K8,62		K30
ubiquitination	K13,22,64	K9,13,69	K13,49,59
Glycosylation	S7,53,62,6	S7,11,15,	S7,17,62
	6	T61,71	T62
Glycation	22	9,22,23,49	22,52
Subcellular localization	C, PN >Na	C, PN >N	C, PN >N	NTb	NTb	NTb	NTb	NTb	NTb	NTb	NTb	NTb	NTb
NES	31–37	30–37	33–37	33–37	25–36					25–36

The ExPASy bioinformatics program was used to calculate the theoretical pI and molecular mass values (Bjellqvist et al., 1994).

^aC=cytoplasmic, PN=perinuclear, N=nuclear; > means more prominent.

^bNT=not tested.