Table 1.
List of primer and their sequences used for PCR to generate constructs.
| Primers | Sequence |
|---|---|
| R1_EcoRI+ | CCTCAGTAAATGAATTCTTCG |
| R1_XhoI- | CCACCTTCTCGAGTACATCG |
| Slippery+ | CAAATCCCTTGAGGACTTCTAGGCGGCCCACTCAGCTTTC |
| Slippery- | GGCCGCCTAGAAGTCCTCAAGGGATTTGAACCCAGC |
| A1+3380 | TGCAGTTTTCAGGTTCC |
| M4 | GTTTTCCCAGTCACGAC |
| dSLB-5′+ | AAGAGGGGAACAACAGTAAAATTGCAAAAAATAGAG |
| dSLB-5′- | GCAATTTTACTGTTGTTCCCCTCTTGCAACTCG |
| dSLB-3′+ | CCCTGTTGGCAATAGGAGTAGTTCCCGTACCC |
| dSLB-3′- | ACTACTCCTATTGCCAACAGGGTCGGCGAG |
| dSLC-5′+ | AATAGAGTGCGACCCTGGGAAACAGGTACC |
| dSLC-5′- | CCCAGGGTCGCACTCTATTTTTTGCAATTTTACTG |
| dSLC-3′+ | GTGCGCACGTTTTTCTTTTAGGTAGGAGCAC |
| dSLC-3′- | CCTAAAAGAAAAACGTGCGCACAACCACACAGAGG |
| dSLD-5′+ | GTTATTTCCTTACCTCTGGTAAAACAAAATTGGC |
| dSLD-5′- | ACCAGAGGTAAGGAAATAACTACAACAGTGAG |
| dSLD-3′+ | AGAGGGCGCAAACTCAGGTTAATAAAACAG |
| dSLD-3′- | AACCTGAGTTTGCGCCCTCTGGAGCAAGTGC |
| dSLC-5′a+ | AATAGAGTGCGCGGGAGCAAGACCCTACTAC |
| dSLC-5′a- | CTTGCTCCCGCGCACTCTATTTTTTGCA |
| dSLC-5′b+ | TCCCGTACCCCAGTAGACGAACCGGCATCG |
| dSLC-5′b- | GTTCGTCTACTGGGGTACGGGAACTACTCCTAG |
| dSLC-5′c+ | GACCCTACTAGACCCTGGGAAACAGGTACC |
| dSLC-5′c- | GTTTCCCAGGGTCTAGTAGGGTCTTGCTCCCGC |
| dSLC-3′a+ | GTGCGCACGTCTGTTGTAGTTATTTCCTTTTTC |
| dSLC-3′a- | ACTACAACAGACGTGCGCACAACCACACAG |
| dSLC-3′b+ | GAAGACTCTCATTTTCTTTTAGGTAGGAGCAC |
| dSLC-3′b- | CTAAAAGAAAATGAGAGTCTTCCGACAACGAC |
| SLC sSL5′b+ | GAGTGCTAGGAGTAGTTGGGGTACCCGCGGGAGCAAGACCC |
| SLC sSL5′b- | GTCTTGCTCCCGCGGGTACCCCAACTACTCCTAGCACTCTAT |
| SLC sSL3′b+ | GGAGTAGTTCCCGTACCCGCCCCAGCAAGACCCTACTACAGTAG |
| SLC sSL3′b- | CTGTAGTAGGGTCTTGCTGGGGCGGGTACGGGAACTACTCC |
| SLC sSLmLoop+ | GCTAGGAGTAGTTCCCGTAGGCGCGGGAGCAAGACCCTACTAC |
| SLC sSLmLoop- | GTAGGGTCTTGCTCCCGCGCCTACGGGAACTACTCCTAGC |
| SLC sSLmR+ | GAGTGCTAGGAGTAGTTGGGGTACCCGCCCCAGCAAGACCCTACTACAGTAG |
| SLC sSLmR- | CTGTAGTAGGGTCTTGCTGGGGCGGGTACCCCAACTACTCCTAGCACTCTAT |
| SLC largeSL5′b+ | GCAAAAAATAGAGTGCTACCAGTAGTTCCCGTACCCGCGG |
| SLC largeSL5′b- | GCGGGTACGGGAACTACTGGTAGCACTCTATTTTTTGCAA |
| SLC largeSL3′b+ | CTCACTGTTGTAGTTATTTGGTTTTTCTTTTAGGTAGGAGC |
| SLC largeSL3′b- | GCTCCTACCTAAAAGAAAAACCAAATAACTACAACAGTGAG |
| slipSLm1+ | GGCATCCCGGAAATCAGCCTAGCTGAGAAGCGGGCCAGTAG |
| slipSLm1- | GGCCCGCTTCTCAGCTAGGCTGATTTCCGGGATGCCTAAAATAG |
| slipSLm2+ | GGCATCCCGGAAATCAGAGTAGCTGAGAAGCGGGCCAGTAG |
| slipSLm2- | GGCCCGCTTCTCAGCTACTCTGATTTCCGGGATGCCTAAAATAG |
| SLC sSLmLoop2+ | GCTAGGAGTAGTTCCCGTACTCGCGGGAGCAAGACCCTACTAC |
| SLC sSLmLoop2- | GTAGGGTCTTGCTCCCGCGAGTACGGGAACTACTCCTAGC |
| 3′SLCsSL-2forSP | TCCGACAACGACGTGCGCAC |