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Summary of the RdRp activity of wild and mutant AmCPV S2 encoded protein using poly (A) as a template and Oligo U18 as primer.
| AmCPV S2 encoded protein | Template | Primer | Amount of enzyme (µg) | Incorporation of [32P] UTP (CPM) | Specific activity (pmol/μg of protein/h |
|---|---|---|---|---|---|
| Wild type | Poly (rA) | Oligo U | 2 | 135693 | 13.48 |
| Wild type | Poly (rA) | – | 2 | 954 | |
| Wild type | – | Oligo U | 2 | 587 | |
| Wild type | Poly (rA) | Oligo U | 0 | 241 | |
| Mutant GAD | Poly (rA) | Oligo U | 2 | 1352 | |
| AmCPV S2 | |||||
| Mutant GAA | Poly (rA) | Oligo U | 2 | 808 | |
| AmCPV S2 |
