Table 5.

Primers and probes used for quantitative RT-PCR of ThkPV-1, ThkPV-2 and ThkPV-3.

Strains	Primers/probes	Sequences	Product length
ThkPV-1	Forward	5'-TGTTAAGCCTAGCACACATGCA-3'	69 bp
	Reverse	5'-GTAAGAAAGCAGGCAGCAATTTC-3'
	Probe	5'-[FAM] TACCAAAGCCAGATGAGA [MGB]-3'
ThkPV-2	Forward	5'-GGCGTGCTGCAAGAGAAAA-3'	67 bp
	Reverse	5'-TGTGTCGGATTATTGTTTCTTGATTT-3'
	Probe	5'-[FAM]ATCACTGTAGTCTCACATCT[MGB]-3'
ThkPV-3	Forward	5'-GCAATGTTTGCCAATTCTATGAAC-3'	68 bp
	Reverse	5'-CGTGGGTGTATCCACTCAAAAA-3'
	Probe	5'-[FAM] AACTGTATGGATATCCC [MGB]-3'