Table 1.
Time-course of gene expression related to the unfolded protein response within human neurons infected by HCoV-OC43.a
| Gene name | Common gene name | Gene ID | 24 h post-infection |
48 h post-infection |
72 h post-infection |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Microarray NT2-N | qPCR NT2-N | qPCR LA-N-5 | Microarray NT2-N | qPCR NT2-N | qPCR LA-N-5 | Microarray NT2-N | qPCR NT2-N | qPCR LA-N-5 | |||
| ATF4 | ATF4 | NM_001675 | –b | 1.12 | 1.11 | 3.38 | 3.47 | 1.63 | 2.49 | 2.58 | 3.25 |
| DDIT3 | CHOP | NM_004083 | –b | 1.30 | 1.54 | 3.69 | 4.59 | 6.38 | 2.22 | 5.10 | 12.24 |
| EDEM1 | EDEM1 | NM_014674.1 | –b | 1.20 | 1.13 | -b | 5.54 | 4.56 | –b | 5.00 | 15.38 |
| DNAJB11 | Erdj3 | NM_016306 | –b | 1.14 | 1.13 | 3.49 | 3.20 | 1.91 | 3.02 | 2.64 | 4.22 |
| DNAJB9 | Erdj4 | NM_012328 | 2.07 | 1.34 | 1.16 | 5.02 | 5.45 | 2.73 | 3.25 | 4.63 | 4.42 |
| PPP1R15A | GADD34 | NM_014330.3 | –b | 1.06 | 1.13 | –b | 3.02 | 3.14 | –b | 3.20 | 7.00 |
| HSPA5 | GRP78 | NM_005347.2 | –b | 0.75 | 0.95 | –b | 1.58 | 1.23 | –b | 1.18 | 1.72 |
| HSP90B1 | GRP94 | NM_003299.1 | –b | 1.11 | 1.03 | 6.56 | 3.59 | 2.18 | 4.50 | 3.05 | 3.77 |
| HERPDU1 | HERPDU1 | NM_014685 | 2.08 | 1.55 | 1.87 | 8.88 | 5.83 | 9.06 | 4.23 | 5.13 | 35.10 |
| DNAJC3 | P58-IPK | NM_006260 | –b | 1.54 | 1.16 | 3.56 | 8.09 | 15.85 | –b | 8.51 | 17.51 |
| XBP1 | XBP1(s) | NM_001079539.1 | –b | 2.17 | 2.46 | –b | 13.99 | 15.56 | –b | 5.24 | 26.35 |
a
Numbers represent the degree of induction (fold-induction) of each gene in HCoV-OC43 infected NT2-N or LA-N-5 cells, at the indicated times post-infection, as revealed by either microarray or real-time quantitative PCR (qPCR).
b
Gene is not significantly modulated according to microarray results.