Fig. 7.

The stability of the sgRNA3a constructs used for transfection experiments in barley protoplasts. A. Autoradiogram representing the intensity of bands corresponding to individual radioactive sgRNA3as that were transfected into protoplasts and extracted after incubation for 15, 45, or 75 min. M stands for the size marker; the names of the constructs are indicated on the right hand side. B. The results of densitometric analysis of the bands shown in A, calculated as the percentage of the initial band intensity.