Fig. 8.

Recombination in planta between 3a-truncated BMV RNA3 and sgRNA3a. C. quinoa seedlings were co-inoculated with wt RNAs 1 and 2, B3-33del RNA3, and various amounts of JS-21 sgRNA3a (the molar ratios of JS-21 to B3-33del are indicated on the top, lanes 1–5). Total RNA was extracted from the inoculated leaves seven days later, separated by electrophoresis in a denaturing agarose gel, blotted onto nylon membrane, and probed with the BMV (+) strand 3′ probe (see Materials and methods). BMV RNAs were detected in plants infected with higher molar ratios of JS-21 to B3-33 del RNAs (lanes 3 to 5). Lane 6 shows the migration of marker BMV RNAs extracted from a viral preparation that was isolated from local lesion tissue after infection with wt transcript BMV RNAs. The lower panel shows the concentration of ribosomal RNA (rRNA) after staining with ethidium bromide. The positions of individual BMV RNAs are indicated on the right. The asterisk marks possible degradation product (in necrotic lesions of C. quinoa tissue).