Table 1.
Cell-to-cell fusion mediated by FIV Env mutant glycoproteins.a
| Env glycoprotein | Relative fusion (% wild-type)b |
|---|---|
| Wild-type | 100 |
| Wild-type+AMD3100 (50μg/ml) | 0.3±0.1 |
| C804S | 16.2±2.7 |
| C811S | 7.6±1.4 |
| C815S | 52.2±7.0 |
| C804S/C811S | 0.7±0.3 |
| C811S/C815S | 3.9±0.7 |
| C804S/C811S/C815S | 0.3±0.1 |
a
293T cells expressing the wild-type or the mutant FIV Env glycoproteins together with HIV-1 Tat protein were dissociated and equivalent numbers of cells were added at 1:5 ratio to 4×104 TZM-bl cells. Coculture was continued for 48 h, after which cells were stained for β-galactosidase and scored for syncytia formation. A fraction of the transfected 293T cells was subjected to immunoblotting to monitor Env expression.
b
Data presented were obtained from three independent experiments, and each assay was performed in triplicate. Blue foci were quantitated as described in “Materials and methods”. Values represent means±standard deviations.