Fig. 5.

Autophagy was induced in PAMs by PRRSV infection: (A) and (B) PAMs, prepared and maintained as described in Materials and methods, were inoculated with PRRSV; the total proteins were harvested at indicated time points, and then subjected to Western blotting analysis to detect the changes of LC3-I and LC3-II. Panel A is the result of Western blotting of uninfected PAMs, while panel B is the result of Western Blotting of infected PAMs. (C)–(E) Confocal microscopy images of infected PAMs. PAMs were fixed at 24 h pi and stained with antibodies against N protein of PRRSV (C) and LC3 (D). Panel E is the merged image of panels (C) and (D). Panels (F)–(H) are mock-infected PAMs stained with antibodies against N protein of PRRSV, LC3 and merge. Scale bars in panels (C)–(H) represent 10 μm.