Fig. 3.

Relative IL-1β, IFN-β and -γ mRNA expression in trachea and lungs of chickens infected with the Conn A5968 strain of IBV. (A–C) represents relative IL-1β, IFN-β, and -γ mRNA expression in trachea, respectively. (D–F) represents relative IL-1β, IFN-β, and -γ mRNA expression in the lung, respectively. Experimental design was as indicated in the legend of Fig. 1. Target mRNA expression was normalized via the geometrical mean of Eff.(uninfected control)^Cp for all control genes (β-actin and ubiquitin). Error bars represent SEM. ⁎=relative mRNA expression is significantly up-regulated when compared to the uninfected controls, and ⁎⁎=relative mRNA expression is significantly down-regulated when compared to uninfected controls.