Fig. 4.

Identification of nsp1α and nsp1β expression in transfected cells. HEK293T cells were transfected with either control plasmid, pCAGGS or plasmids expressing PRRSV nsp1 proteins, including pCAGGS, pCAGGS-nsp1α or pCAGGS-nsp1β. At 24 h post-transfection, cells were fixed for immunofluorescence assay or harvested for Western blot analysis. (A) Immunofluorescent detection of nsp1α and nsp1β expression in HEK293T cells. Cells were stained with a specific antibody as indicated at the bottom of the picture, and DyLight 549-conjugated goat anti-rabbit or anti-mouse antibody was used as the secondary antibody. Images were taken by fluorescence and phase-contrast microscopy using a 20× objective. (B) Western blotting analysis nsp1, nsp1α and nsp1β expression in 293T cells. Membranes were probed with an nsp1 protein specific antibody as indicated at the bottom of each membrane. Arrows point to the specific PRRSV proteins.