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. 2009 Dec 16;398(1):87–97. doi: 10.1016/j.virol.2009.11.033

Fig. 5.

Fig. 5

PRRSV nsp1 proteins inhibit IFN-β production. (A, B) HEK293T cells cultured in 24-well plates were cotransfected with a plasmid expressing nsp1 proteins, a plasmid expressing influenza virus NS1, or pCAGGS empty vector (P), pRL-SV40, and a luciferase reporter plasmid p125-Luc (A) or pCIB-55-Luc (B). At 20 h post transfection, cells were infected with Sendai virus (SeV) for 16 h to stimulate the production of interferon. (C–H, J) HEK293T cells in 24-well plates were cotransfected with the plasmid pEFneo-RIG-I (C), pEFneo-MDA5 (D), pEGFP-IPS-1 (E), pEFneo-TBK1 (F), pEFneo-IKKɛ (G), or pCAGGS-IRF3 (H), or pcDNA3-TRIF (J), along with pRL-SV40, pCAGGS expressing nsp1 proteins, and pCIB-55 plasmid for 20–24 h. (I) HEK293T cells were cotransfected with pNF-kB-luc, pcDNA3-TRIF, pRL-SV40 and pCAGGS expressing nsp1 proteins or pCAGGS empty vector (P) for 20 h. Cells were harvested and measured for firefly and Renilla luciferase activities. Relative luciferase activity is defined as a ratio of firefly luciferase reporter activity to Renilla luciferase activity. Each data point shown represents a mean value from three experiments. Error bars show standard deviations of the normalized data.