Fig. 4.
T lymphocytes from B19 birds inoculated with either H5N9 derived HA or NP expression plasmids respond to a heterologous (H7N2) virus. At 8 weeks p.i., T lymphocytes from chickens receiving either HA or NP cloned from the H5N9 strain were co-cultured with APCs infected with H5N9 or H7N2 viruses. The T cell responses are expressed as the average (± S.E.) of NO production for each treatment group. T lymphocytes from plasmid-inoculated chickens had significantly greater responses to H7N2 AIV strain than those from the PBS control group (p ≤ 0.01). PBMC were prepared from three individual chickens for each stimulation assay.