Fig. 4.

A2MC2 replication induces elevated expression of IFN-stimulated genes in MARC-145 cells. (A) Elevation of STAT2 and ISG56 detected by Western blotting. The cells were infected with A2MC2 or UV-inactivated virus at 1 TCID₅₀ per cell, followed by treatment with PPMO 5UP1 to inhibit A2MC2 replication, and at 24 hpi, treated with or without IFN-α. Cell lysate from uninfected cells was included as a control. (B) Elevation of IFN-β, ISG15 and ISG56 expression detected by real-time PCR. Treatment of the cells with IFN-α was included as a control. Relative induction folds in comparison with mock-treated cells are shown. Error bars represent variation between repeated experiments. Significant differences between A2MC2-infected cells and the uninfected cells are denoted by “*”, which indicates a P-value of<0.05. (C) Kinetics of STAT2 and ISG56 expression in MARC-145 cells infected with different MOIs of A2MC2. The cells were harvested at 24, 48 and 72 hpi for Western blot amalyses. Samples of uninfected cell lysates were included as controls.