Table 1.
Primers used to construct the full-length IBV clone
| Clone | Primer | Nucleotide sequence | Location |
|---|---|---|---|
| A | T7-IBV | 5′-CGCTAGCTAATACGACTCACTATAGGACTTAAGATAGATATTAATA-3′ | 1–20 |
| IBV-5753R | 5′-CGGGATCCGTCTCGCGACAACACTCTTAAC-3′ | 5737–5753 | |
| B | IBV-5748F | 5′-ATTATGGTCTCTGTCGCTAGCTATAAGACCG-3′ | 5748–5768 |
| IBV-8694R | 5′-GGGTCTCGGCCTCAAATTTATCACCTATC-3′ | 8673–8694 | |
| C | IBV-8689F | 5′-CGGGATCCGGTCTCGAGGCCTACCTTTCAGCG-3′ | 8689–8706 |
| IBV-15532R | 5′-GCAAAAGGTCTCAATGAATCAC-3′ | 15,511–15,532 | |
| D | IBV-15511F | 5′-CGGGATCCGTGATTCATTGAGACCTTTTGC-3′ | 15,511–15,532 |
| IBV-20930R | 5′-ACACCTGCAGATGTAACATC-3′ | 20,911–20,930 | |
| E | IBV-20887F | 5′-GTTTACACCTCTAATGAGACCATAG-3′ | 20,887–20,911 |
| IBV-27608R | 5′-GGAATTCGGTCTCG(T)30TGCTCTAACTCTATACTAGC-3′ | 27,589–27,608 |