Fig. 3.

Involvement of cathepsin L in Eco-MLV vector infection. (A) NIH3T3 and mCAT1-expressing TE671 (TE671/mCAT1) cells were pretreated with a cathepsin L inhibitor, CLIK148. Transduction titers of the Eco-MLV vector were measured in the treated cells. Relative values to titers in cells treated with equal volume of DMSO are indicated. (B) Numbers of viable cells were measured, and relative values to numbers of viable cells in DMSO-treated cells are indicated. (C) Transduction titers of VSV-G-pseudotyped MLV vector in the pretreated cells were measured. Relative values to titers in DMSO-treated cells are indicated. These experiments were repeated three times. Error bars indicate standard deviations. Asterisks indicate significant differences compared with values in DMSO-treated cells.