Fig. 4.

Effects of CLIK148 on cathepsin B and L activities in NIH3T3 and TE671 cells. (A) NIH3T3 and TE671 cells were incubated with the cathepsin L detection reagent for 0 or 1 h, and MFIs of the cells were measured by a flow cytometer. Relative values to MFIs of cells incubated for 0 h are indicated. (B and C) Cathepsin L activities of CA-074Me- or CLIK148-treated cells were measured by the cathepsin L detection reagent. Histograms are indicated in panel (B). Closed areas indicate cells treated with DMSO and unstained with the cathepsin L detection reagent. Open areas indicate cells treated with DMSO and stained with the reagent. Grey areas indicate cells treated with CLIK148 (middle panel) or CA-074Me (lower panel) and stained with the reagent. Relative values to MFIs of DMSO-treated cells are also indicated in panel (C). (D) Cathepsin B activity of CLIK148-treated cells was measured by the cathepsin B detection reagent. Relative values to MFIs of DMSO-treated cells are indicated in the left panel. Histogram was indicated in the right panel. Closed area indicates cells treated with DMSO and stained with the cathepsin B detection reagent. Red line indicates cells treated with CLIK148 and stained with the reagent. These experiments were repeated three times. Error bars indicate standard deviations. Asterisks indicate significant differences compared to values in the control cells.