Fig. 4.

RV infection activates CaMKI which leads to G1 to S phase progression. (A) Whole cell lysates isolated from MA104 cells infected with SA11 for indicated time points were subjected to western blot analysis using CaM, pCaMKI and GAPDH (loading control) specific antibody. Results are representative of three independent experiments. (B) MA104 cells were kept mock infected or infected with SA11 strain in presence or absence of W7 and BAPTA-AM for 2–8 h or kept mock infected followed by propidium iodide staining and cell cycle analysis using flow cytometer. Each bar represent % of cell present in specific phase of cell cycle. Results are representative (mean±SD) of three independent experiments. (C) MA104 cells were kept mock infected or infected with SA11 strain in presence or absence of W7 and BAPTA-AM for 2–8 h or kept mock infected followed by western blot analysis using pCaMKI and GAPDH (loading control) specific antibody. Results are representative of three independent experiments.