Table 4.
Heterophil phagocytosis and oxidative burst1 in 7.5 wk old Hy-Line Brown pullets fed for 3.5 wk (Experiment 1) or 14 wk old pullets fed for 4 wk (Experiment 2) on diets containing different types of fiber (Mean, pooled SEM, n = 8).
| Treatment | C2 | MF3 | IF4 | Pooled SEM |
|---|---|---|---|---|
| Experiment 1 | ||||
| PI (latex beads/100 cells) | 303.4a | 328.5a,b | 350.3b | 7.0 |
| %P (% cells containing 1 or more beads) | 81.4 | 82.1 | 83.8 | 1.0 |
| PE (PI:%P) | 3.7a | 4.0b | 4.2b | 0.1 |
| Oxidative burst (ΔRFU)5 | 3,509.0a | 4,330.0b | 5,264.4c | 184.8 |
| Experiment 2 | ||||
| PI (latex beads/100 cells) | 376.4a | 403.5a,b | 447.9b | 10.8 |
| %P (% cells containing 1 or more beads) | 75.4 | 79.4 | 82.3 | 1.5 |
| PE (PI:%P) | 5.0a | 5.1a,b | 5.5b | 0.1 |
| Oxidative burst (ΔRFU)5 | 744.1a | 1,302.9b | 1,857.7b | 135.6 |
a–cMeans within the same row with different superscripts differ significantly (P < 0.05).
1After 1 h incubation with phorbol 12-myristate 13-acetate.
2Control diet - see Table 1 for diets used in Experiment 1 and Experiment 2.
3MF mixed soluble and insoluble fiber Control diet plus 1.0% (Experiment 1) or 1.5% (Experiment 2) OptiCell C5.
4IF insoluble fiber diet Control diet plus 1.0% (Experiment 1) or 1.5% (Experiment 2) Arbocel RC fine.
PE, phagocytosis efficiency; PI, phagocytosis index; %P, phagocytosis percentage.
5ΔRFU Change in relative fluorescent unit, see methods for calculation.