Table 9.10.
The Helsinki poliovirus experiment (Ranta et al., 2001)
| Helsinki population = 740,000 Sewage flow 2 × 108 l/day Contamination Event 5 × 1010 TCID50 Poliovirus vaccine Flushed down toilet in one liter volume 20 km from sewage treatment plant Detection Automatic sampler = 200 ml per 5 × 106 liters of sewage flow Four samples pooled per day Concentrated from 400 to 1 ml before assay Result Virus was detected for the next 4 days (cell culture) Peak 24–48 h after flush Virus detected after passage of 800 million liters of sewage pass through system Conclusion Monitoring of sewage could detect 1 infected person in 10,000 Assumes:
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