Fig. 2.

EBOV-GP-driven entry is CatB/CatL-dependent irrespective of the target cell line. Equal volumes of VSVpp bearing the indicated glycoproteins or bearing no glycoprotein were used for transduction of 293T, Huh-7, A549, Calu-3, Vero E6, and EpoNi/22.1 cells pre-incubated for 3 h with E-64d in the indicated concentrations or DMSO as control. At 24 h post transduction, luciferase activities in cell lysates were quantified as indicator of transduction efficiency. Transduction mediated by EBOV-GP and VSV-G is shown relative to transduction mediated by control particles bearing no glycoprotein, which was set as 1. The average of four independent experiments conducted with separate pseudotype preparations is shown. Error bars indicate SEM. One-way ANOVA with Bonferroni post-test analysis was performed to test statistical significance (*, p ≤ 0.05).