Fig. 3.

Calu-3 cells are largely resistant to entry driven by filoviral but not arena-, paramyxo-and rhabdoviral glycoproteins. Vero E6 and Calu-3 cells were transduced with equal volumes of VSV particles pseudotyped with the glycoproteins from diverse viruses (A) or pseudotyped with filovirus glycoproteins (B). Particles bearing no glycoprotein served as negative control. At 24 h post transduction, luciferase activities in cell lysates were quantified as indicator of transduction efficiency. Transduction mediated by the different glycoproteins is shown relative to transduction mediated by control particles bearing no glycoprotein, which was set as 1. The average of three independent experiments is shown. Error bars indicate SEM. (C) Huh-7 and Calu-3 cells were inoculated with trVLPs encoding nano-luciferase for 6 h, washed three times and incubated for 48 h. Thereafter, the luciferase activity (given as relative luminescence units, RLU) in cell lysates was measured. The averages of four (Huh-7) and six (Calu-3) independent experiments are shown, error bars indicate SEM. Two-way ANOVA with Sidak posttest analysis was performed to test statistical significance (**, p ≤ 0.01; ***, p ≤ 0.001).