Fig. 1.

Coronavirus infection of porcine alveolar macrophages (PAMs) and lung-derived fibroblast-like cells from ANPEP wild-type (WT) and knockout (KO) pigs. (A, C) PAMs and lung fibroblasts were infected with PDCoV at an MOI of 0.1; the infected cells were fixed and stained with AlexaFluor488-labeled anti-PDCoV antibody and nuclei were counterstained with propidium iodide (PI) at two days after infection. (B, D) PAMs and lung fibroblasts were infected with TGEV at an MOI of 1; the infected cells were fixed and stained with AlexaFluor488-labeled anti-TGEV antibody and nuclei were counterstained with PI at 48 h post-infection. Representative pictures are shown from experiments performed on cells derived from three WT and three KO pigs.