Table 1.
RT-PCR for PDCoV nucleic acid in feces and virus neutralizing activity in seruma.
| Pig | ANPEP Genotype | RT-PCR |
Virus Neutralization | |||
|---|---|---|---|---|---|---|
| Day after infection | ||||||
| 1 | 2 | 3 | 4 | |||
| 18 | Wild-Type | 38.1 | >40 | >40 | >40 | 1/64 b |
| 19 | Wild-Type | 35.2 | 37.3 | >40 | >40 | <16 |
| 22 | Wild-Type | 36.6 | 38.1 | >40 | >40 | ND c |
| 20 | Knockout | 37.1 | 36.0 | >40 | >40 | 1/64 |
| 21 | Knockout | 35.1 | 37.1 | >40 | >40 | <16 |
| 23 | Knockout | 32.5 | 32.5 | 36.3 | >40 | ND |
a
Ct > 40, negative for detectable quantities of PDCoV nucleic acid. Virus neutralization assays were performed at 14 days after infection. A tite <1/16 was considered negative for detectable neutralizing activity. All pigs at day 0 were negative for neutralizing activity.
b
Neutralizing activity was measured on day 10 after infection.
c
ND, Not Done.