Table 2.
Experimental design of mouse and pigs immunization.
| Group | Inoculum | Routes | Immunization dose | Immunization days | |
|---|---|---|---|---|---|
| Mouse | 1 | VSVMT-S | IN | 103 PFU | 0, 10 |
| 2 | VSVMT-S | IN | 102 PFU | 0, 10 | |
| 3 | VSVMT-S | IM | 103 PFU | 0, 10 | |
| 4 | VSVMT-S | IM | 102 PFU | 0, 10 | |
| 5 | PBS | IM | / | 0, 10 | |
| Pig | 1 | VSVMT-S | IN | 107 PFU | 0, 10 |
| 2 | VSVMT-S | IN | 106 PFU | 0, 10 | |
| 3 | VSVMT-S | IM | 107 PFU | 0, 10 | |
| 4 | VSVMT-S | IM | 106 PFU | 0, 10 | |
| 5 | PBS | IM | / | 0, 10 | |
a. IN: intranasal inoculation.
b. IM: intramuscular injection, viruses were mixed with complete or incomplete Freund's adjuvant at ratio of 1:1.