Table 3.
Viral shedding of experimental piglets after SADS-CoV inoculation analyzed by qPCR. +, positive; -, negative. CT value of 35 is considered as the threshold. DPI, days post-inoculation.
| DPI | SADS-CoV/GDWT-P7-inoculated group |
SADS-CoV/GDWT-P83-inocualted group |
Mock-infected gourp |
|||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A1 | A2 | A3 | A4 | A5 | A6 | A7 | A8 | A9 | B1 | B2 | B3 | B4 | B5 | B6 | B7 | B8 | B9 | C1 | C2 | C3 | C4 | C5 | C6 | C7 | C8 | |
| 0 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 |
| 1 | +/23.91 | +/28.27 | +/34.47 | +/25.87 | +/24.72 | +/32.74 | +/26.62 | +/25.94 | +/25.20 | -/40.00 | -/38.95 | -/40.00 | -/40.00 | -/39.01 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/39.94 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 |
| 2 | +/26.27 | +/29.09 | +/30.32 | +/31.53 | +/30.13 | -/37.94 | +/26.86 | +/26.91 | +/29.93 | -/38.85 | -/40.00 | +/31.38 | -/40.00 | -/40.00 | -/40.00 | -/38.36 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/38.52 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 |
| 3 | +/28.66 | +/27.28 | +/32.26 | +/27.71 | +/31.08 | -/38.36 | +/32.68 | +/25.21 | +/28.36 | +/33.35 | +/34.91 | +/33.08 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/38.18 | -/36.63 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 | -/40.00 |
| 4 | +/31.44 | / | -/38.64 | +/29.09 | / | -/40.00 | +/28.61 | / | +/29.89 | / | -/40.00 | -/37.39 | +/34.01 | / | +/34.13 | / | -/38.49 | +/34.39 | -/40.00 | / | -/37.78 | -/40.00 | / | -/40.00 | -/40.00 | / |
| 5 | +/28.06 | / | -/40.00 | +/26.73 | / | -/40.00 | +/23.95 | / | +/26.00 | / | -/40.00 | +/29.25 | -/40.00 | / | -/38.34 | / | -/40.00 | -/40.00 | -/40.00 | / | -/40.00 | -/38.07 | / | -/40.00 | -/40.00 | / |
| 6 | +/31.97 | / | -/40.00 | +/34.72 | / | -/40.00 | +/26.91 | / | +/27.66 | / | -/40.00 | -/40.00 | +/33.48 | / | -/40.00 | / | -/37.39 | -/38.42 | -/40.00 | / | -/38.54 | -/38.39 | / | -/38.29 | -/40.00 | / |
| 7 | -/40.00 | / | -/38.31 | -/40.00 | / | -/40.00 | -/37.26 | / | +/34.60 | / | -/40.00 | -/40.00 | -/40.00 | / | -/40.00 | / | -/38.39 | -/40.00 | -/40.00 | / | -/40.00 | -/40.00 | / | -/40.00 | -/37.95 | / |
| 8 | -/40.00 | / | -/40.00 | -/40.00 | / | -/40.00 | -/40.00 | / | -/40.00 | / | -/40.00 | -/40.00 | -/40.00 | / | -/40.00 | / | -/40.00 | -/40.00 | -/40.00 | / | -/40.00 | -/40.00 | / | -/38.01 | -/40.00 | / |
| 9 | -/40.00 | / | -/40.00 | -/40.00 | / | -/40.00 | -/38.67 | / | -/38.95 | / | -/40.00 | -/40.00 | -/40.00 | / | -/40.00 | / | -/40.00 | -/40.00 | -/40.00 | / | -/40.00 | -/40.00 | / | -/40.00 | -/40.00 | / |
/, A2, A5, A8, B1, B5, B7, C2, C5 and C8 were euthanized and necropsied for collecting tissue samples.