Fig. 3.

APN and EGFR synergistically promote TGEV invasion. (A and B) APN and EGFR interference verification, shAPN1 and shEGFR3 were later used in the subsequent experiments. (C) IPEC-J2 cells were transfected with interference vector pLVX-shRNA-APN, pLVX-shRNA-APNCtrl, pLVX-shRNA-EGFR, pLVX-shRNA-EGFRCtrl, or pLVX-shRNA-APN + pLVX-shRNA-EGFR through lentiviral supernatant. Normal cells served as controls. Cells were infected with TGEV at an MOI of 2, and cultured for 1 h. The invasion of TGEV was detected by RT-PCR. (D and E) IPEC-J2 cells were infected with Dylight 488-TGEV, and cultured for 1 h The invasion of TGEV was detected by Flow cytometry. (F) The viral titers of intracellular TGEV were analyzed by TCID₅₀. (G) Intracellular TGEV was analyzed by viral plaque morphology in ST cells. The data shown are the mean results ± SD from three independent experiments. (* 0.01 < p < 0.05, ** p < 0.01).