Fig. 7.

EGFR internalization through clathrin endocytosis pathway. (A) IPEC-J2 cells were transfected with interference vector pLVX-shRNA-Clathrin3 or pLVX-shRNA-Caveolin2 through lentiviral supernatant. Normal cells served as controls. Cells were infected with TGEV at an MOI of 2. The protein of the cell membrane was extracted. Cell membrane EGFR was analyzed by Westernblot using rabbit anti-EGFR pAb. (B) The ratio of EGFR to the mean of E-cadherin and GAPDH was normalized to control conditions. The data shown are the mean results ± SD from three independent experiments.