Table 2.
The siRNA sequence (sense strand) in this study
| Name | Target gene | siRNA target sequence | Positiona |
|---|---|---|---|
| siDNApol | DNA polymerase gene (dnapol) | 5′-ATTGACGAGTGCAATCAGATT-3′ | 2920–2940 |
| siRR2 | Ribonucleotide reductase small subunit gene (rr2) | 5′-CGTCCGAATTGAACAAGAATT-3′ | 849–869 |
| siTK-TMK | Thymidine kinase and thymidylate kinase gene (tk-tmk) | 5′-TACTTTTGGTGTCGAAAGATT-3′ | 1020–1040 |
| siVP24 | vp24 | 5′-AATGCTACAGGAAGGGAATTT-3′ | 433–453 |
| siVP28 | vp28 | 5′-GATTAACCCATCAAAGGCCTT-3′ | 426–446 |
| siMUTb | Mutant | 5′-GATTACAAACGCAAAGGCCTT-3′ |
a
Positions of siDNApol, siRR2, siTK-TMK, siVP24 and siVP28 target sequences were according to the WSSV genome (GenBank accession no. AF332093).
b
Mutated siRNA (siMUT) has six mismatch nucleotides siVP28.