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. 2014 Aug 9;464:274–286. doi: 10.1016/j.virol.2014.07.027

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Copyright © 2014 Elsevier Inc. All rights reserved.

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Fig. 2 — Stability of PRRSV GP5 domains in rTGEV vectors. (A) Schematic representation of PRRSV GP5 constructs: full-length GP5 (GP5), GP5 ectodomain (GP5ecto), and GP5 fragment (GP5fr) that comprises the ectodomain lacking the signal peptide (SP). Immunodominant epitope (IDE) and epitope critical in neutralization (ECN), N-glycosylation sites (yellow), and the cysteine involved in GP5-M heterodimer formation (red) are also shown. GP5ecto and GP5fr included an HA or FLAG tag, respectively, for their detection (TG, blue). (B) RT-PCR analysis of ten clones from plaque-purified passage 8 rTGEV-S_7.1-TRS_3a-GP5-TRS_22N-M (GP5), rTGEV-S_7.1-TRS_3a-GP5ecto-TRS_22N-M (GP5ecto) and rTGEV-S_7.1-TRS_3a-GP5fr-TRS_22N-M (GP5fr) viruses. The arrow indicates the expected size of the corresponding PCR product. Numbers on the left indicate the molecular weight markers (Mw) size in base pairs. Lower size bands (indicated by red asterisks) correspond to deletion products from heterologous gene, meaning genomic instability. Numbers on the right indicate the overall stability of each construct.