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. 2014 Aug 9;464:274–286. doi: 10.1016/j.virol.2014.07.027

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Copyright © 2014 Elsevier Inc. All rights reserved.

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Fig. 3 — Expression of PRRSV minor envelope protein domains by rTGEV vectors. (A) Schematic representation of PRRSV GP3 and GP4 proteins, and corresponding GP3fr and GP4fr expressed by rTGEV. Several motifs are indicated, such as signal peptide (SP), the epitope critical in neutralization (ECN), the transmembrane domain (TM), the glycosylation sites (yellow), and immunodominant epitope (IDE). GP3fr and GP4fr included a FLAG tag (FG) for their detection. (B) RT-PCR analysis of ten clones from plaque-purified passage 16 rTGEV-S_7.1-TRS_3a-GP3fr (GP3fr) and rTGEV-S_7.1-TRS_3a-GP4fr (GP4fr) viruses. Genomic RNA (gRNA) and GP3fr and GP4fr subgenomic mRNAs (mRNA) were detected. The arrow indicates the expected size of the corresponding PCR product. Numbers on the left indicate the molecular weight markers (Mw) size in base pairs.