Table 1.
Structure of PRRSV fusion proteins expressed by rTGEV. Amino acids in each segment forming PRRSV fusion proteins.
| Fusion proteina | Segment 1b | Segment 2 | Segment 3 | Segment 4 |
|---|---|---|---|---|
| GP3fr | GP3 (1–28) | GP3 (51–77) | FLAGc | – |
| GP4fr | GP4 (1–25) | GP4 (51–75) | FLAG | – |
| GP5fr | GP5 (35–68) | FLAG | – | – |
| GP3ep-Mloop | M (1–66) | GP3 (51–77) | FLAG | M (67–173) |
| GP3ep-NtermM | FLAG | GP3(51–77) | M (1–173) | – |
a
PpuMI (AGGTCCT) and BlpI (GCTCGAGC) restriction sites were introduced at the 5′ and 3′ ends, respectively. An optimized Kozak sequence (GCCACC) was placed immediately upstream the ATG start codon to improve protein translation efficiency.
b
The numbers in brackets indicate the amino acids included in the construct.
c
All constructs included the FLAG tag sequence (amino acids DYKDDDDK).