Fig. 1.
Organization of an EV71 genome and polyprotein processing. (A) The 5′ and 3′UTR contain RNA sequences forming RNA structural elements. The coding region is divided into three sections, including the P1, P2 and P3 precursor. Protein 3AB is derived from the P3 precursor of enteroviral polyprotein, which also contains 3DPol and 3C protease (3CPro). 3AB will be proteolytically cleaved into proteins 3A and 3B/VPg. (B) Electrophoresis analysis of purified MBP-3AB and MBP alone. Proteins were loaded onto a 10% SDS-PAGE gel and then visualized via Coomassie blue staining. Lane 1, protein molecular mass markers; lane 2, purified MBP; lane 3, purified MBP-3AB.