Table 1.
The list of Primers.
| Primers | Sequence (5′-3′) |
|---|---|
| Primers | |
| 3AB-F | GAATTCGGTCCACCTAAGTTCA (EcoR I) |
| 3AB-R | CTCGAGTTACTGTACTGTTGCTGTGCGAA (Xho I) |
| 3A F | GAATTCGGTCCACCTAAGTTCA (EcoR I) |
| 3A R | CTCGAGTTACTGAAACCCTGCAAAGA (Xho I) |
| 3B F | GAATTCGGTGCGTATTCTGGT (EcoR I) |
| 3B R | CTCGAGTTACTGTACTGTTGCTGT (Xho I) |
| 3B+7-F | GAATTCAAGCTCTTTGCAG (EcoR I) |
| 3B+7-R | CTCGAGTTACTGTACTGTTGCTGT (Xho I) |
| K80A-F | GAATTCgcaCTCTTTGCAGGGTT (EcoR I) |
| K80A-R | CTCGAGTTACTGTACTGTTGCTGT (Xho I) |
| F82A-F | GAATTCAAGCTCgcaGCAGGGTTTCA (EcoR I) |
| F82A-R | CTCGAGTTACTGTACTGTTGCTGT (Xho I) |
| F85A-F | GAATTCAAGCTCTTTGCAGGGgcaCAGGGTGCGTAT (EcoR I) |
| F85A-R | CTCGAGTTACTGTACTGTTGCTGT (Xho I) |
| Y89A-F | GAATTCAAGCTCTTTGCAGGGTTTCAGGGTGCGgcaTCTGGTGCTC (EcoR I) |
| Y89A-R | CTCGAGTTACTGTACTGTTGCTGT (Xho I) |
| R103A-F | GAATTCAAGCTCTTTGCAG (EcoR I) |
| R103A-R | CTCGAGTTACTGTACTGTTGCTGTtgcAAGAGCAGGTTTC (Xho I) |
Underlined characters indicate restriction endonuclease sites; types are shown in parentheses. Substituted nucleotides for mutagenesis are shown with lowercase characters.