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Release of PEDV in polarized epithelial cells. PEDV was used to infect either polarized Vero E6 at an MOI of 0.5 or IECs at an MOI of 5, and 24 h later, the apical and the basolateral medium were collected for extracting RNA. Real-time PCR was performed to determine the copies of PEDV genomic RNA. All data are expressed as mean±SD. ⁎p<0.05; ⁎⁎p<0.01.
