Fig. 1.

Inhibition of pAPN-recoginzing peptides on cell infection by PEDV. (A) PEDV (PFU=5×10³/ml) was first incubated with Vero cells at 37 °C for 1 h, followed by the addition of various concentrations of peptides H, S or F. (B) Vero cells were incubated with various concentrations of peptides H, S or F at 37 °C for 1 h, then infected with PEDV at an PFU of 5×10³/ml. (C) Peptides H, S or F were first incubated with PEDV at 37 °C for 1 h, and then the peptide treated viruses (PFU=5×10³/ml) were used to infect Vero cells at 37 °C. Plaque assays were performed at the end of each experiment. Serially-diluted polyclonal antibody against PEDV and PBS were used as positive and negative controls, respectively. Peptide concentrations 1, 2, 3, 4, and 5 are 250 μg/ml, 125 μg/ml, 62.5 μg/ml, 31.25 μg/ml, and 15.625 μg/ml, respectively. Anti-PEDV antibody dilutions 1, 2, 3, 4, and 5 are 1:4, 1:8; 1:16; 1:32; and 1:64, respectively. Bars show the standard deviation from three independent assays.